CST, 74471S, SARS-CoV-1 Membrane Protein Antibody

Polyclonal Antibody for studying Membrane Protein SARS-CoV. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-1 Membrane Protein Antibody (CST #74471) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-1 Membrane Protein Antibody recognizes transfected levels of total SARS-CoV-1 membrane protein. This antibody does not cross-react with SARS-CoV-2 or MERS-CoV membrane proteins. Species Reactivity: Virus Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly211 of SARS-CoV-1 membrane protein. Antibodies are purified by peptide affinity chromatography. Background The cause of the SARS epidemic in 2003 was a novel pathogenic coronavirus, originally termed SARS-CoV (severe acute respiratory syndrome coronavirus) (1). Following the 2019 emergence of SARS-CoV-2 and the COVID-19 pandemic, SARS-CoV is now often referred to as SARS-CoV-1. SARS-CoV-1 is a member of the Coronaviridae family of single-stranded RNA viruses (2). The genome of SARS-CoV-1 is relatively large among viruses (~30 kDa); it encodes four key structural proteins (spike, envelope, membrane, and nucleocapsid) that make up the virion particle, in addition to a variety of smaller open reading frames (ORFs) that encode the accessory proteins required for viral replication (3). The SARS-CoV-1 membrane protein (M) is the most abundant protein in the viral envelope; in addition to its structural role, it plays an important role in virus-host interactions and the viral lifecycle (4). Notably, studies have shown that membrane proteins from both SARS-CoV-1 and SARS-CoV-2 may induce apoptosis in host cells, suggesting one mechanism by which SARS coronaviruses cause tissue damage upon infection (5,6). Alternate Names E1 glycoprotein; M protein; Matrix glycoprotein; matrix protein; Membrane glycoprotein; membrane glycoprotein M; Membrane protein; SARS-CoV membrane protein; sars5; VME1_CVHSA Specification REACTIVITY: Vir SENSITIVITY: Transfected Only MW (kDa): 20-30 SOURCE: Rabbit