CST, 74499T, TBL1XR1/TBLR1 (D4J9C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TBL1XR1. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, TBL1XR1/TBLR1 (D4J9C) Rabbit Monoclonal Antibody (CST #74499) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity TBL1XR1/TBLR1 (D4J9C) Rabbit Monoclonal Antibody recognizes endogenous levels of total TBL1XR1/TBLR1 protein. This antibody also cross-reacts with an unidentified protein of 130 kDa. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro159 of human TBL1XR1/TBLR1 protein. Background Transducing β-like protein 1 (TBL1X/TBL1) and TBL1-related protein 1 (TBL1XR1/TBLR1) were originally identified as subunits of the co-repressor silencing mediator for retinoic and thyroid hormone receptor (SMRT) and nuclear receptor co-repressor (NCoR) complexes (1-3). These two factors are required for the exchange of co-repressor complexes for co-activators by acting as adaptors to recruit the ubiquitin/proteasome machinery that degrades the co-repressor proteins during ligand mediated activation of transcription (4,5). Co-factor exchange driven by TBL1X/TBL1 and TBL1XR1/TBLR1 appears to be the mechanism by which c-Jun and NF-κB mediated transcription is activated and is therefore likely to be the mechanism employed by other signal-dependent transcription factors as well (4,6). In addition, both TBL1X/TBL1 and TBL1XR1/TBLR1 have essential roles in regulating the Wnt-signaling pathway by recruiting β-catenin to Wnt target genes to activate transcription. Depletion of TBL1X-TBL1XR1 significantly inhibited Wnt-beta-catenin- induced gene expression and oncogenic growth in vitro and in vivo (7). Research studies have shown that upregulation of TBL1XR/TBLR1 is observed in a variety of solid tumors, and is correlated with advanced tumor stage, metastasis and poor prognosis (1). Alternate Names C21; DC42; F-box-like/WD repeat-containing protein TBL1XR1; FLJ12894; IRA1; MRD41; nuclear receptor co-repressor/HDAC3 complex subunit; Nuclear receptor corepressor/HDAC3 complex subunit TBLR1; TBL1-related protein 1; TBL1R; TBL1X receptor 1; TBL1XR1; TBLR1; transducin (beta)-like 1 X-linked receptor 1; transducin beta like 1 X-linked receptor 1; Transducin beta-like 1X-related protein 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 50, 60 Source/Isotype: Rabbit IgG