CST, 74558S, GNG4 (E5O7M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GNG4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GNG4 (E5O7M) Rabbit Monoclonal Antibody (CST #74558) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GNG4 (E5O7M) Rabbit Monoclonal Antibody recognizes endogenous levels of total GNG4 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val49 of human GNG4 protein. Background G protein-coupled receptors (GPCRs) transmit extracellular signals through heterotrimeric G proteins (guanine nucleotide-binding proteins) (1). G proteins have three main subunits: Gα, Gβ, and Gγ. In the quiescent state, the guanosine diphosphate (GDP) bound Gα is associated with Gβγ. Binding of ligands to GPCR causes the exchange of GDP for guanosine triphosphate (GTP) on Gα, leading to its dissociation from the Gβγ complex (2). The released GTP-Gα further activates downstream effectors to initiate signaling events. G protein-mediated signaling is terminated by hydrolysis of GTP by Gα intrinsic GTP hydrolase and reassociation of Gα with Gβγ to form the inactive heterotrimer. G protein subunit gamma 4 (GNG4) is a member of the G protein γ family and plays a role in transducing signals from GPCRs (3,4). In humans and mice, 14 γ G protein subunits have been identified (5). GNG4 plays critical roles in guanosine GDP synthesis, guanosine triphosphatase (GTPase) activity, and the regulation of G protein-effector interactions (6,7). has been reported to act as a tumor suppressor gene in glioblastoma (8). Expression of GNG4 has been identified as an immunotherapy marker associated with colon cancer prognosis, and is associated with multiple malignant phenotypes of lung adenocarcinoma (9,10). Alternate Names DKFZp547K1018; FLJ23803; FLJ34187; G protein subunit gamma 4; G-gamma 4; GBG4; GNG4; GNGT4; guanine nucleotide binding protein (G protein), gamma 4; guanine nucleotide binding protein 4; Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 9 Source/Isotype: Rabbit IgG