CST, 74560T, p14 ARF (E3X6D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying p14 Arf. Validated for WB,IHC,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, p14 ARF (E3X6D) Rabbit Monoclonal Antibody (CST #74560) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31426 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity p14 ARF (E3X6D) Rabbit Monoclonal Antibody recognizes endogenous levels of total p14 ARF protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro126 of human p14 ARF protein. Background Human p14 ARF (p19 ARF in mouse) is a pro-apoptotic cell cycle regulator frequently inactive in human tumors (1). Basal expression of p14 ARF is low in most cell types, but accumulation of this protein occurs in response to oncogene expression (2,3). Increased p14 ARF levels facilitate MDM2 degradation, leading to increased p53 protein levels and subsequent cell cycle arrest and/or apoptosis (4). While most p14 ARF signaling has traditionally thought to be p53-dependent, more recent reports have described p53-independent p14 ARF signaling leading to cell cycle arrest and apoptosis (5,6). Alternate Names Alternative reading frame; ARF; CD2A2; CDK4 inhibitor p16-INK4; CDK4I; CDKN2; CDKN2A; cell cycle negative regulator beta; CMM2; cyclin dependent kinase inhibitor 2A; cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); Cyclin-dependent kinase inhibitor 2A, isoform 4; cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3; cyclin-dependent kinase inhibitor p16; INK4; INK4a; MLM; MTS-1; MTS1; multiple tumor suppressor 1; p14; p14ARF; p16; p16-INK4; p16-INK4a; p16-INK4A iso5; p16INK4; p16INK4a; p19; p19ARF; TP16; Tumor suppressor ARF Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Rabbit IgG