CST, 74698S, SARS-CoV-2 Envelope Protein Antibody

Polyclonal Antibody for studying Envelope Protein SARS-CoV-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 Envelope Protein Antibody (CST #74698) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-2 Envelope Protein Antibody recognizes endogenous levels of total SARS-CoV-2 envelope protein in SARS-CoV-2-infected cells. Species Reactivity: Virus Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of SARS-CoV-2 envelope protein. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Envelope protein is transcribed and translated from nested subgenomic RNA of the SARS-CoV-2 genome. Despite the SARS-CoV-2 envelope protein being very small, it possesses a number of functionalities that are critical for viral assembly and release. It is also a major virulence factor, a viroporin that contributes to the host immune mediated cytokine storm (3). A C-terminal motif on envelope protein binds to and mislocates a number of cell tight junction proteins in lung epithelial cells which may contribute to alveolar damage and acute respiratory distress syndrome (ARDS) (4). Alternate Names 2019-nCoV envelope protein; E protein; envelope protein; envelope small membrane protein; SARS-CoV-2 envelope protein; severe acute respiratory syndrome coronavirus 2 envelope protein; sM protein; VEMP_SARS2 Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 12 SOURCE: Rabbit