CST, 74736S, Phospho-RRM2 (Thr33) Antibody

Polyclonal Antibody for studying RRM2 (Thr33) phosphate mouse. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-RRM2 (Thr33) Antibody (CST #74736) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-RRM2 (Thr33) Antibody recognizes endogenous levels of RRM2 protein only when phosphorylated at Thr33. By western blot, this antibody detects a 110 kDa band of unknown origin. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr33 of mouse RRM2 protein. Antibodies are purified by peptide affinity chromatography. Background Ribonucleotide reductase catalyzes the rate-limiting step in the synthesis of deoxynucleotide triphosphates (dNTPs). Ribonucleoside-diphosphate reductase subunit M2 (RRM2) is frequently overexpressed and associated with poor prognosis in multiple human cancers (1). RRM2/AKT/NF-κB signaling pathway is implicated in tumor invasiveness in gastric cancer (2). RRM2 is highly expressed in melanoma, and correlated with poor prognosis in BRAF-mutant melanoma. Knockdown of RRM2 stabilized the transient response of cells and patient-derived xenograft (PDX) model system to BRAF inhibition (3). Cyclin-dependent kinase (CDK) mediated phosphorylation of RRM2 at Thr33 targets the protein for degradation, allowing cells to maintain balanced dNTP pools (4). Alternate Names AA407299; MGC113712; R2; ribonucleoside-diphosphate reductase M2 chain; Ribonucleoside-diphosphate reductase subunit M2; ribonucleotide reductase M2; Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit; RIR2; Rrm2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 45 SOURCE: Rabbit