CST, 74766SF, IFITM3 (D8E8G) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying IFITM3. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, IFITM3 (D8E8G) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #74766) is ready to ship. Product Usage Information This product is the carrier free version of product #59212. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 59212 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity IFITM3 (D8E8G) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total IFITM3 protein. This antibody does not cross-react with IFITM1 or IFITM2 proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val5 of human IFITM3 protein. Background Interferon-induced transmembrane protein (IFITM) family members are composed of short amino- and carboxy-termini, two transmembrane domains, and a cytoplasmic domain (1). There are four family members in humans: IFITM1, IFITM2, IFITM3, and IFITM5 (2,3). Mice have two additional family members, IFITM6 and IFITM7 (2,3). Basal expression of IFITM proteins is observed in some cells and expression can also be induced by type I and type II interferons (4-6). The primary function of IFITM family proteins appears to be viral restriction, as IFITM proteins inhibit cytosolic entry of viruses by preventing fusion of viral and host membranes (7,8). The mechanism by which IFITM proteins inhibit fusion is unclear. Although IFITM proteins are present on both the plasma membrane and intracellular membranes, they most effectively restrict viral fusion in late endosomes and lysosomes (8,9). In addition, different family members exhibit specific viral preferences (9). For example, IFITM3 is most effective at restricting influenza A infection, while IFITM1 is more successful in controlling filoviruses and SARS (9,10). Alternate Names 1-8U; Dispanin subfamily A member 2b; DSPA2b; IFITM3; IFM3; interferon induced transmembrane protein 3; interferon induced transmembrane protein 3 (1-8U); Interferon-induced transmembrane protein 3; Interferon-inducible protein 1-8U; IP15 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG