CST, 74775S, Pontin/RUVBL1 (D1L8J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Pontin/RUVBL1. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, Pontin/RUVBL1 (D1L8J) Rabbit Monoclonal Antibody (CST #74775) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity Pontin/RUVBL1 (D1L8J) Rabbit Monoclonal Antibody recognizes endogenous levels of total Pontin/RUVBL1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu428 of human Pontin/RUVBL1 protein. Background Reptin/RuvBL2 and Pontin/RuvBL1 are closely related members of the AAA+ (ATPase associated with diverse cellular activities) superfamily of proteins, and are putatively homologous to bacterial RuvB proteins that drive branch migration of Holliday junctions (1). Reptin and Pontin function together as essential components of chromatin remodeling and modification complexes, such as INO80, TIP60, SRCAP, and Uri1, which play key roles in regulating gene transcription (1,2). In their capacity as essential transcriptional co-regulators, Reptin and Pontin have both been implicated in oncogenic transformations, including those driven by c-Myc, β-catenin, and E1A (2-7). Alternate Names 49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP-interacting protein; 54 kDa erythrocyte cytosolic protein; ECP-54; ECP54; epididymis secretory sperm binding protein; INO80 complex subunit H; INO80H; NMP 238; NMP238; Nuclear matrix protein 238; PONTIN; Pontin 52; Pontin52; RuvB (E coli homolog)-like 1; RuvB like AAA ATPase 1; RuvB-like 1; RuvB-like 1 (E. coli); RuvB-like AAA ATPase; RUVB1; RUVBL1; RVB1; TAP54-alpha; TATA binding protein interacting protein 49 kDa; TIH1; TIP49; TIP49A; TIP60-associated protein 54-alpha Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG