CST, 74807T, SIGMAR1 (D7L1M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SIGMAR1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SIGMAR1 (D7L1M) Rabbit Monoclonal Antibody (CST #74807) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SIGMAR1 (D7L1M) Rabbit Monoclonal Antibody recognizes endogenous levels of total SIGMAR1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro70 of human SIGMAR1 protein. Background Sigma non-opioid intracellular receptor 1 (SIGMAR1) is an endoplasmic reticulum (ER) membrane chaperone that forms raft-like microdomains on the ER, where it interacts with mitochondria at the mitochondria-associated ER membrane domain (MAM). At MAM, SIGMAR1 maintains proper ER-mitochondrion Ca2+ signaling, regulates mitochondria function, and enhances cellular survival upon ER stress (1-4). When activated, SIGMAR1 translocates to ER and plasma membrane, where it interacts with a plethora of membrane proteins, including ion channels, neurotransmitter receptors, and kinases. SIGMAR1 also modulates a variety of neuronal functions, such as neuronal excitability, neuroplasticity, neuroprotection, and neurorestoration (5-7). SIGMAR1 binds to many anti-psychotic drugs and it is implicated in addiction, pain, neurodegenerative diseases, and depression (8-11). Recently, mutations in the gene have been reported to be associated with amyotrophic lateral sclerosis (12,13). Besides its important roles in central nervous system and peripheral nervous system, SIGMAR1 also enhances cancer cell migration and invasion (14,15). Alternate Names aging-associated gene 8 protein; ALS16; DSMA2; FLJ25585; hSigmaR1; MGC3851; opioid receptor, sigma 1; OPRS1; OTTHUMP00000000515; OTTHUMP00000000516; OTTHUMP00000000517; OTTHUMP00000021286; Q99720; Q99720-2; Q99720-5; SGMR1; SIG-1R; Sigma 1-type opioid receptor; Sigma non-opioid intracellular receptor 1; sigma non-opioid intracellular receptor 1 isoform 1; sigma1 receptor; Sigma1-receptor; Sigma1R; SIGMAR1; SR-BP; SR-BP1; SR31747 binding protein 1; SR31747-binding protein; SRBP; type I sigma receptor Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG