CST, 74875SF, XRCC1 (E4A3V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying XRCC1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, XRCC1 (E4A3V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #74875) is ready to ship. Product Usage Information This product is the carrier free version of product #76998. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 76998 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity XRCC1 (E4A3V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total XRCC1 protein. In some cell lysates, this antibody detects a 62 kDa band of unknown origin by western blot. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro623 of human XRCC1 protein. Background The X-ray repair cross-complementing protein 1 (XRCC1) is a DNA repair protein important in both single strand break repair and base excision repair following damage from ionizing radiation and alkylating agents (1). XRCC1 acts as a scaffold protein to coordinate DNA abasic site repair through interaction with several other repair proteins (2). At least eight XRCC1 protein partners have been identified, including the polynucleotide kinase PNK (3), DNA ligase III (4,5), poly (ADP-ribose) polymerase (6), and PCNA (7). Mutations and polymorphisms in the gene serve as diagnostic markers and are associated with elevated risk of various forms of cancers (8). Alternate Names DNA repair protein XRCC1; RCC; SCAR26; X-ray repair complementing defective repair in Chinese hamster cells 1; X-ray repair cross complementing 1; X-ray repair cross complementing protein 1; X-ray repair cross-complementing protein 1; X-ray-repair, complementing defective, repair in Chinese hamster; XRCC1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 82 Source/Isotype: Rabbit IgG