CST, 74978T, MUC5B (E4Y5Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MUC5B. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, MUC5B (E4Y5Z) Rabbit Monoclonal Antibody (CST #74978) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:150 - 1:600 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 24470 . Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MUC5B (E4Y5Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total MUC5B protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MUC5B protein. Background Mucins are a family of macromolecules that line and protect the respiratory epithelium from microbes and pollutants in the local environment. Of the family members that are known to date, some are produced in a cell type and tissue-specific manner, suggesting distinct biological roles for members. Some members polymerize after secretion to form gel-like substances that coat the epithelial layer. MUC5AC and MUC5B are family members that polymerize in this manner. Others do not polymerize, and others yet, have a transmembrane domain and remain physically attached to the epithelia (1). While it is known that mucins are protective to the respiratory epithelium, it has been reported that changes in expression of mucins are associated with several forms of lung disease, such as cystic fibrosis, COPD, asthma, pulmonary fibrosis, and others (1-4). Multiple studies have shown that one of the largest known risks of developing lung fibrosis is linked to a genetic variant of MUC5B (5,6). Alternate Names Cervical mucin; cervical mucin MUC5B; high molecular weight mucin; High molecular weight salivary mucin MG1; MG1; MUC-5B; MUC5; MUC5B; MUC9; mucin 5, subtype B, tracheobronchial; mucin 5B, oligomeric mucus/gel-forming; Mucin-5 subtype B, tracheobronchial; Mucin-5B; mucin, salivary (high molecular weight); Sublingual gland mucin Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG