CST, 74986T, Mm47 (F4E5V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Mm47 mouse. Validated for WB,IP,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Mm47 (F4E5V) Rabbit Monoclonal Antibody (CST #74986) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:100 - 1:200 Immunofluorescence (Immunocytochemistry): 1:400 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Mm47 (F4E5V) Rabbit Monoclonal Antibody recognizes endogenous levels of total Mm47 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse Mm47 protein. Background Many mammalian genomes contain previously unannotated short open reading frames (sORFs) that potentially code for small, functional proteins termed micropeptides (1,2,5). Micropeptides have recently been shown to play roles in cellular processes, including differentiation, proliferation, and apoptosis (2,3,5-7). One of these micropeptides, short transmembrane protein 1 (STMP1, Mm47), localizes at the inner mitochondrial membrane and has increased expression in various cancers. Mm47 is associated with metastasis and recurrence of hepatocellular carcinoma (1,2,4). Genetic studies show that Mm47 promotes mitochondrial fission and increased migration of tumor cells (1,2,4). Overexpression of Mm47 leads to redistribution of mitochondria to the leading edge of cells and enhanced lamellipodia formation (2,4). Mm47 silencing inhibits tumor metastasis in xenograft mouse models (2). Collectively, recent studies highlight Mm47 as an important regulator of metastasis and an important player in mitochondrial fission, providing a potential therapeutic target. Alternate Names 1810058I24Rik; AI414869; AI848234; lncRNA 1810058I24Rik; Mitochondrial micropeptide-47; Mm47; RIKEN cDNA 1810058I24 gene; Short transmembrane mitochondrial protein 1; STMP1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 9-15 Source/Isotype: Rabbit IgG