CST, 75017SF, NY-ESO-1 (D1Q2U) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CTAG1B. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, NY-ESO-1 (D1Q2U) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #75017) is ready to ship. Product Usage Information This product is the carrier free version of product #45437. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 45437 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity NY-ESO-1 (D1Q2U) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total NY-ESO-1 protein. This antibody cross-reacts with NY-ESO-2/LAGE-1S. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NY-ESO-1 protein, isoform 1. Background Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3). New York esophageal squamous cell carcinoma-1 (NY-ESO-1) is an X-linked CTA and was first identified by serological analysis of cDNA expression libraries in esophageal carcinoma (SEREX) (4,5). Like other CTAs, NY-ESO-1 expression is repressed in normal somatic tissues but becomes derepressed in a variety of human cancer types, such as multiple myeloma, non-small cell lung carcinoma, liposarcoma, and melanoma (6,7). Although the biological function of NY-ESO-1 remains enigmatic, its tumor-restricted expression pattern and high degree of immunogenicity have positioned it as a prominent target of immunotherapy-based strategies for tumor eradication (8). Alternate Names Autoimmunogenic cancer/testis antigen NY-ESO-1; cancer antigen 3; Cancer/testis antigen 1; cancer/testis antigen 1B; Cancer/testis antigen 6.1; CT6.1; CTAG; CTAG1; CTAG1A; CTAG1B; CTG1B; ESO1; L antigen family member 2; LAGE-2; LAGE2; LAGE2A; LAGE2B; New York esophageal squamous cell carcinoma 1; NY-ESO-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 20 (monomer), 40 (dimer) Source/Isotype: Rabbit IgG