CST, 75028SF, Ly-6G (E6Z1T) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Ly6g mouse. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Ly-6G (E6Z1T) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #75028) is ready to ship. Product Usage Information This product is the carrier free version of product #87048. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 87048 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Ly-6G (E6Z1T) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Ly-6G protein. This antibody does not cross-react with Ly-6C1, Ly-6C2, Ly-6F, or Ly-6I. Non-specific staining was observed in mouse gastrointestinal epithelium by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with mouse Ly-6G recombinant protein. Background The Ly-6 complex is a series of genes found on chromosome 15. These genes code for a number of different proteins that can be used as surface markers. The family members vary in their biologic expression and have been shown to be involved in cell signaling and cell adhesion (1). The structure of these proteins includes a motif known as the LU domain that has three loops comprised of disulfide bonds. These bonds are formed by 8 to 10 cysteines that can cause differences in the length of the loops as well as the sequences at each tip (2,4). There are 11 known Ly-6 genes on murine chromosome 15 that code for different proteins. These family members, excluding secreted Ly6/Plaur domain containing 1 coded by the gene, are attached to the cell surface by a GPI anchor near the C terminus. The structure of these proteins may play a role in transmembrane interactions, and downstream signaling cascades (1,2). Ly-6 proteins have been widely used as differentiation markers on hematopoietic cells. The ability to isolate and express specific Ly-6 antibodies through hybridoma technology has allowed researchers to identify unique proteins (1). These proteins are expressed on subsets of immune cells at different stages of development, such as T cells, B cells, monocytes, granulocytes, and macrophages (1-5). ~Ly-6G is used as a marker for mouse neutrophils (2,3). It is also expressed by mouse polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) (6). Alternate Names Gr-; Gr-1; Gr1; Ly-6; Ly-6G; ly-6G.1; Ly6g; lymphocyte antigen 6 complex, locus G; Lymphocyte antigen 6G; neurotoxin homologue Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 20-40 Source/Isotype: Rabbit IgG