CST, 75243S, PKN3 (F6S5C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PKN3. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, PKN3 (F6S5C) Rabbit Monoclonal Antibody (CST #75243) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PKN3 (F6S5C) Rabbit Monoclonal Antibody recognizes endogenous levels of total PKN3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val882 of human PKN3 protein. Background Protein Kinase N3 (PKN3) is a PKC-related protein kinase that acts downstream of Rho GTPases and PI3K, supporting tumor invasion, metastasis, and angiogenesis (1-3). PKN3 is highly expressed in cancer cells, and silencing PKN3 expression is under investigation as a means of treating human cancers (4). Atu027, a therapeutic siRNA that silences PKN3 expression in the vascular endothelium, has been shown to prevent or delay metastasis in human pancreatic cancer (5). Alternate Names PKN3; PKNBETA; protein kinase N3; Protein kinase PKN-beta; Protein-kinase C-related kinase 3; RP11-545E17.1; Serine/threonine-protein kinase N3; UTDP4-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG