CST, 75330T, Maoa (E3L3B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MAOA mouse. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Maoa (E3L3B) Rabbit Monoclonal Antibody (CST #75330) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:800 - 1:3200 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity Maoa (E3L3B) Rabbit Monoclonal Antibody recognizes endogenous levels of total Maoa protein. This antibody recognizes mouse Maoa protein and is also reactive with human MAOA protein; however, this antibody is not suggested for immunohistochemical analysis of human tissues. Nonspecific staining was observed in mouse testis by immunohistochemistry. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala467 of mouse Maoa protein. Background Monoamine oxidase A (MAOA), a mitochondrial enzyme, catalyzes the degradation of monoamines. Studies show that increased expression of MAOA promotes prostate cancer metastasis by activating the Shh-IL6-RANKL signaling network (1). In addition, MAOA expression in adipose tissue macrophages is upregulated in aging, leading to the reduction of noradrenaline availability in adipose tissue. Decreased levels of noradrenaline contribute to the aging-related decline of catecholamine-induced lipolysis in adipocytes (2). Furthermore, sympathetic neuron-associated macrophages import and degrade norepinephrine via the transporter SLC6A2 and MAOA, respectively, and thus contribute to obesity (3). Alternate Names 1110061B18Rik; AA407771; Amine oxidase [flavin-containing] A; AOFA; MAO-A; Maoa; MGC27811; monoamine oxidase A; Monoamine oxidase type A; OTTMUSP00000018396 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG