CST, 75357S, VPS26A Antibody

Polyclonal Antibody for studying VPS26A. Validated for Western Blotting. Highly specific and rigorously validated in-house, VPS26A Antibody (CST #75357) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity VPS26A Antibody recognizes endogenous levels of total VPS26A protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro221 of human VPS26A protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Vacuolar protein sorting-associated protein 26A (VPS26A), together with VPS29 and VPS35, is part of a trimeric protein complex known as the cargo-selective complex (CSC) (1). The CSC is regarded as the core functional component of the retromer, a multimeric protein complex involved in selective transport of cargo proteins from endosomes to the trans-Golgi network or plasma membrane (2). As part of the CSC, VPS26A does not have intrinsic membrane-binding activity but relies on association with RAB7A for recruitment to the cytosolic face of the endosomal membrane (3,4). Retromer defects are associated with neurological disease, and VPS26A mutations have been linked to perturbed endosomal cargo sorting in atypical parkinsonism (5). Alternate Names FLJ12930; HB58; Hbeta58; hVPS26; PEP8A; vacuolar protein sorting 26 homolog A; vacuolar protein sorting 26 homolog A (S. pombe); Vacuolar protein sorting-associated protein 26A; Vesicle protein sorting 26A; VP26A; VPS26; VPS26 retromer complex comonent A; VPS26, retromer complex component A; VPS26A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 38 SOURCE: Rabbit