CST, 75390S, PROS1 (E7F5H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PROS1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PROS1 (E7F5H) Rabbit Monoclonal Antibody (CST #75390) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PROS1 (E7F5H) Rabbit Monoclonal Antibody recognizes endogenous levels of total PROS1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PROS1 protein. Background PROS1 (PROS, Vitamin K-dependent protein S) is a vitamin K-dependent functional protein. It has an N-terminal Gla domain containing multiple Asp carboxylation sites, followed by four EGF repeats, and two C-terminal LG domains. Vitamin K mediates multiple carboxylations of glutamic acid residues in the PROS1 Gla domain. These modifications are required for PROS1 to exert its functions (1). PROS1 is well known for its anticoagulant role during thrombosis (2,3). The anticoagulation function of PROS1 is exerted by both intrinsic and extrinsic mechanisms. Extrinsically, PROS1 functions as a cofactor in the TFPI (tissue factor pathway inhibitor) to inhibit Factor Xa generation and prevent coagulation (4). Intrinsically, PROS1 functions as a cofactor for activated protein C (aPC) (4). PROS1-aPC promotes degradation of Factors V and VIII, and prevents coagulation and thrombin formation (5). Another important function of PROS1 is as a ligand activator of the TAM (Tyro3, Axl, and MerTK) RTK family receptor. Among the TAM family members, PROS1 binds and activates Tyro3 and MerTK (6-8). The ligand/receptor interactions activate multiple downstream signaling pathways, such as STAT, ERK, Akt, NF-kB, Bcl-2, and FAK (9). PROS1/TAM interactions play important roles in immune and nervous inflammation and homeostasis, cancer development, and immune-related multiple sclerosis (9-11). Alternate Names PROS; PROS1; protein S; protein S (alpha); protein Sa; PS21; PS22; PS23; PS24; PS25; PSA; THPH5; THPH6; vitamin K-dependent plasma protein S; Vitamin K-dependent protein S Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 78, 80 Source/Isotype: Rabbit IgG