CST, 75432T, BCL11A (D4E3P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Bcl-11A. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BCL11A (D4E3P) Rabbit Monoclonal Antibody (CST #75432) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BCL11A (D4E3P) Rabbit Monoclonal Antibody recognizes endogenous levels of total BCL11A protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val247 of human BCL11A protein. Background BCL11A is a zinc finger-containing transcriptional repressor that is important for normal hematopoiesis (1). Alternative splicing of the BCL11A transcript results in several isoforms of the protein (2). BCL11A is required for the early stages of B lineage commitment and mice lacking BCL11A fail to develop B cells (1). Mice deficient in BCL11A also fail to develop plasmacytoid dendritic cells (3). In addition, BCL11A regulates the switch from fetal to adult hemoglobin by repressing expression of fetal hemoglobin in adult erythroid cells (4). Since expression of fetal hemoglobin can decrease the severity of hemoglobin disorders in adults, BCL11A is a potential therapeutic target for these diseases (4). BCL11A was also recently identified as a component of the mammalian SWI/SNF complex (5). BCL11A is required for morphogenesis and terminal differentiation of dorsal spinal neurons (6). Alternate Names B cell CLL/lymphoma 11A; B-cell CLL/lymphoma 11A; B-cell CLL/lymphoma 11A (zinc finger protein); B-cell lymphoma/leukemia 11A; BAF chromatin remodeling complex subunit BCL11A; BC11A; BCL-11A; BCL11A; BCL11A B-cell CLL/lymphoma 11A (zinc finger protein); BCL11A-L; BCL11a-M; BCL11A-S; BCL11A-XL; BCL11A, BAF complex component; C2H2-type zinc finger protein; COUP-TF-interacting protein 1; CTIP1; DILOS; ecotropic viral integration site 9 homolog; Ecotropic viral integration site 9 protein homolog; EVI-9; EVI9; FLJ10173; FLJ34997; HBFQTL5; KIAA1809; Zinc finger protein 856; ZNF856 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 100-145 Source/Isotype: Rabbit IgG