CST, 75442S, ENPP3/CD203c Antibody

Polyclonal Antibody for studying ENPP3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, ENPP3/CD203c Antibody (CST #75442) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ENPP3/CD203c Antibody recognizes endogenous levels of total ENPP3/CD203c protein. This antibody does not cross-react with either ENPP1 or ENPP2 proteins. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ENPP3/CD203c protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3/CD203c/PD-Ιbeta) is a type-II transmembrane glycoprotein that contains a large extracellular domain, an α-helical transmembrane segment, and a short cytoplasmic domain containing the N-terminus. ENPP3 has been shown to be overexpressed in colon carcinoma and is thought to play a role in tumor initiation and tumor cell invasiveness (1-3). Within the hematopoietic cell compartment, ENPP3 is a cell surface marker of basophil and mast cell lineages (4,5). Indeed, ENPP3 is overexpressed on transformed mast cells in patients with systemic mastocytosis (6). Recently, ENPP3 has been identified as being highly overexpressed in renal cell carcinoma (RCC) and may have potential as a novel therapeutic target for this disease (7). Alternate Names Alkaline phosphodiesterase I; B10; CD203c; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); E-NPP 3; ectonucleotide pyrophosphatase/phosphodiesterase 3; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3; ENPP3; gp130RB13-6; MAFP; major acidic fibroblast growth factor stimulated phosphoprotein; NPP3; NPPase; Nucleotide diphosphatase; Nucleotide pyrophosphatase; PD-Ibeta; PDNP3; Phosphodiesterase I beta; Phosphodiesterase I/nucleotide pyrophosphatase 3; phosphodiesterase-I beta Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 140 SOURCE: Rabbit