CST, 7547S, Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying 4E-BP1 (Thr37/Thr46) phosphate. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit Monoclonal Antibody (PE Conjugate) (CST #7547) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit Monoclonal Antibody (PE Conjugate) detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody conjugate may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1. Background Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). Alternate Names 4E-BP1; 4EBP1; BP-1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC4316; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1 Specification REACTIVITY: H M R Mk Dm SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG