CST, 75484T, TIM-4 (D3W4F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TIM-4. Validated for WB,IHC,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, TIM-4 (D3W4F) Rabbit Monoclonal Antibody (CST #75484) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:100 Immunohistochemistry (Paraffin): 1:100 Flow Cytometry (Live): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 27337 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Flow Cytometry (Live) Specificity / Sensitivity TIM-4 (D3W4F) Rabbit Monoclonal Antibody recognizes endogenous levels of total TIM-4 protein. This antibody does not cross-react with TIM-1 or TIM-3. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro215 of human TIM-4 protein. Background T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-4 is expressed by dendritic cells and macrophages (1). Like TIM-1, TIM-4 is a receptor for phosphatidylserine (2). Interaction of TIM-4 with phosphatidylserine exposed by cells undergoing apoptosis leads to uptake of apoptotic cells (2,3). In this way, TIM-4 regulates adaptive immunity by removing apoptotic antigen-specific T cells following clearance of infection. Peritoneal macrophages from mice deficient in TIM-4 fail to effectively clear apoptotic cells leading to hyperactive T and B cells and autoimmunity (4). In addition, TIM-4 expressed by tumor-associated macrophages and dendritic cells leads to autophagy-mediated degradation of dying tumor cells, resulting in reduced antigen presentation and T cell responses (5). TIM-4 was also identified as a ligand for TIM-1, and interaction between these proteins regulates T cell proliferation (1). Finally, TIM-4 is expressed by a subset of proinflammatory B cells that produce IFN-γ and promote Th1 cell differentiation (6). Alternate Names FLJ27515; SMUCKLER; T cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; TIM-4; TIM4; TIMD-4; TIMD4 Specification REACTIVITY: H SENSITIVITY: Endogenous (IHC-P), Transfected (W, F) MW (kDa): 40-60 Source/Isotype: Rabbit IgG