CST, 75550S, gamma-Catenin (D9M1Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Catenin-gamma. Validated for WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, gamma-Catenin (D9M1Q) Rabbit Monoclonal Antibody (CST #75550) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63528 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity gamma-Catenin (D9M1Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total γ-Catenin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro725 of human γ-Catenin protein. Background Also known as plakoglobin, γ-catenin is a member of the Armadillo family of signaling molecules, which includes β-catenin and the protein armadillo (1). This family of proteins is involved in Wnt signaling, which is important in embryonic development and in tumorigenesis (2-3). Although the two vertebrate proteins β- and γ-catenin display sequence homology, γ-catenin likely plays a role distinct from that of β-catenin (1, 4-6). γ-catenin localizes to desmosomes and adherens junctions, both sites of intercellular adhesion, and interacts with the cytoplasmic domains of classical and desmosomal cadherins. Interaction of γ- or β-catenin with α-catenin, desmoplakin and other junction proteins provides a link between intercellular junctions and the actin and intermediate filament cytoskeleton. Maintenance and/or modification of this link is vital for control of cell adhesion and migration (1). γ-catenin is modified by phosphorylation, affecting both adhesion and β-catenin dependent transcription (7), and by and O-glycosylation, affecting adhesion (8). Recent evidence suggests that γ-catenin regulates desmosomal adhesion in response to growth factor stimulation (9). Alternate Names ARVD12; catenin (cadherin-associated protein), gamma (80kD); catenin (cadherin-associated protein), gamma 80kDa; Catenin gamma; CTNNG; Desmoplakin III; Desmoplakin-3; DP3; DPIII; gamma-catenin; Junction plakoglobin; JUP; PDGB; PKGB; PLAK Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 83 Source/Isotype: Rabbit IgG