CST, 75587SF, OLFM4 (D1E4M) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying OLFM4. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, OLFM4 (D1E4M) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #75587) is ready to ship. Product Usage Information This product is the carrier free version of product #14369. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14369 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity OLFM4 (D1E4M) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total OLFM4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe94 of human OLFM4 protein. Background Olfactomedin-4 (OLFM4, hGC-1) is a member of the Olfactomedin family, a small group of extracellular proteins defined by the presence of a conserved "Olfactomedin domain" that is thought to facilitate protein-protein interactions (1). OLFM4 is a secreted glycoprotein, which forms disulfide bond-mediated oligomers, and is thought to mediate cell adhesion through its interactions with extracellular matrix proteins such as lectins (2). Human was first cloned from myeloid cells (3) and is expressed in a distinct subset of neutrophils, though the functional significance of this differential expression pattern remains unclear (4). Among normal tissues, the expression of OLFM4 protein is most abundant in intestinal crypts (5), where it has garnered attention as a possible marker of intestinal stem cells (6). Notably, OLFM4 expression is markedly increased in several tumor types, including colorectal, gastric, pancreas, lung, and breast (reviewed in [1]). Furthermore, research studies show that the expression levels of OLFM4 vary in relation to the severity and/or differentiation status of multiple tumor types (1, 6-8), leading to the suggestion that OLFM4 may have utility as a prognostic marker in some cancer patients (9). Alternate Names Antiapoptotic protein GW112; bA209J19.1; G-CSF-stimulated clone 1 protein; GC1; GW112; hGC-1; hOLfD; KIAA4294; olfactomedin 4; Olfactomedin-4; OlfD; OLFM4; OLM4; UNQ362 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 75-78 Source/Isotype: Rabbit IgG