CST, 75689S, Goat Anti-Mouse IgG2b Antibody (AP Conjugate)

Secondary Antibody for studying in the research area. Product Usage Information Recommended Antibody Dilutions: 1:2000 - 1:4000 Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability. Storage Supplied in 50 mM Tris, 1 mM MgCl2, pH 8.0, and 50% glycerol containing < 0.1% sodium azide. Store at -20°C. Stable for 12 months when stored at -20°C. Do not aliquot the antibody . Specificity / Sensitivity Goat Anti-Mouse IgG2b Antibody (AP Conjugate) detects the heavy chain of mouse IgG2b. This antibody conjugate has been cross-adsorbed against mouse IgG1, IgG2a, IgG3, IgM, and IgA, human, bovine, and horse serum proteins. This antibody conjugate may cross-react with immunoglobulins from other species. Species Reactivity: Mouse Source / Purification Goat Anti-Mouse IgG2b Antibody (AP Conjugate) is produced by immunizing goats with mouse IgG2b. The Anti-Mouse IgG2b antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Background The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on Western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time. Specification REACTIVITY: M Source/Isotype: Goat