CST, 75706S, PREX1 (D8D2K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PREX1. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, PREX1 (D8D2K) Rabbit Monoclonal Antibody (CST #75706) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:150 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity PREX1 (D8D2K) Rabbit Monoclonal Antibody recognizes endogenous levels of total PREX1 protein by immunohistochemistry. This antibody is not recommended for western blot due to weaker signal in comparison to other antibodies available against PREX1. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His770 of human PREX1 protein. Background Phosphoinositide-3,4,5-triphosphate (PtdIns(3,4,5)P )-dependent Rac exchanger 1 (PREX1) is a Rac-specific GTP-exchange factor (GEF) regulated by heterotrimeric G-protein β/γ subunits and the lipid second messenger PtdIns(3,4,5)P (1-4). PREX1 contains two DEP (Dishevelled, Egl-10, and Pleckstrin homology) domains that coordinate heterotrimeric G-protein signaling. It also contains a Dbl-homology domain, which exhibits Rac-GEF activity, and PH and PDZ domains for interacting with upstream and downstream signaling components (1). Originally shown to modulate cellular migration of neutrophils by Rac2 activation (5-8), it is clear that PREX1 plays a broader role in modulating cell migration. PREX1 promotes metastasis of prostate cancer and melanoma cells, affects endothelial junction integrity, and is required for platelet generation and function (9-14). Research studies suggest that PREX1 plays an essential role in mediating ErbB-dependent signaling events in breast cancer by coordinating Rac activation in response to paracrine signals within the tumor microenvironment. Activation of PREX1 downstream of ErbB3 and EGFR chemokine receptors (CXCR4) promotes Rac activation, increased migration, proliferation, tumorigenesis, and metastasis in breast cancer cells (15,16). Consistent with this observation, deletion of PREX1 expression in mice results in resistance to melanoma metastasis (11). Expression of PREX1 in human tumors transplanted into mice inversely correlates with increased tumor progression and poor survival (15). Additional research studies suggest that PREX Rac-GEF activity is enhanced by phosphorylation in response to growth factors or hormones, and may require coincident dephosphorylation of two PH domain serine residues. The upstream kinases and precise regulatory mechanism remains elusive (15,17). Alternate Names KIAA1415; P-Rex1; Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein; phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1; phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1; PIP3 dependent Rac exchange factor 1; PREX1; PtdIns(3,4,5)-dependent Rac exchanger 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 190, 110 Source/Isotype: Rabbit IgG