CST, 75723SF, PSA/KLK3 (D2A8) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PSA (Prostate Specific Antigen). Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),ELISA+. Highly specific and rigorously validated in-house, PSA/KLK3 (D2A8) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #75723) is ready to ship. Product Usage Information This product is the carrier free version of product #5877. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 5877 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PSA/KLK3 (D2A8) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total PSA/KLK3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe110 of human PSA/ KLK3 protein. Background Kallikrein 3 (KLK3), also known as Prostate Specific Antigen (PSA), is a member of the glandular kallikrein subfamily of serine proteases (1). It is produced by prostate epithelial cells and is secreted into prostatic ducts. Upon cleavage of 7 amino-terminal amino acids (2), it is activated to liquefy semen in the seminal coagulum. Although PSA/KLK3 is produced in healthy individuals, investigators have found abnormally high levels in the blood of men with advanced prostate cancer (2,3). Alternate Names APS; Gamma-seminoprotein; hK3; kallikrein related peptidase 3; Kallikrein-3; kallikrein-related peptidase 3; KLK2A1; KLK3; P-30 antigen; prostate specific antigen; Prostate-specific antigen; PSA; Semenogelase; Seminin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG