CST, 75743S, TIM-3 (E9K5D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TIM-3 mouse. Validated for IF,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, TIM-3 (E9K5D) Rabbit Monoclonal Antibody (CST #75743) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Flow Cytometry (Live): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity TIM-3 (E9K5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total TIM-3 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with mouse TIM-3 recombinant protein. Background T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation (1-3 ). TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer (4,5), and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype (5). Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells (6). Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced anti-tumor responses in a mouse model of colorectal cancer (7). In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease (1). Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death (8). Alternate Names CD366; HAVcr-2; Havcr2; HAVR2; hepatitis A virus cellular receptor 2; Hepatitis A virus cellular receptor 2 homolog; MGC124149; MGC124150; OTTMUSP00000005654; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; Tim; TIM-; TIM-3; Tim3; Timd; TIMD-3; Timd3 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG