CST, 75760SF, CEND1 (D6A6) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CEND1. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, CEND1 (D6A6) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #75760) is ready to ship. Product Usage Information This product is the carrier free version of product #8944. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8944 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CEND1 (D6A6) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CEND1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CEND1 protein. Background The progression of progenitor cells towards neuronal differentiation is regulated by cell cycle control and the transition from proliferative to neurogenic cell divisions. Cell cycle exit and neuronal differentiation 1 (CEND1) is a neuronal protein widely expressed in the adult nervous system (1). It is implicated in the synchronization of cell cycle exit and differentiation of neuronal precursors in the developing nervous system, and its expression marks the exit of proliferative cells from the cell cycle (2,3). Levels of CEND1 expression in the subventricular zone of the adult nervous system are critical for cell cycle control and neuronal differentiation mechanisms during neonatal SVZ neurogenesis (4). It has recently been shown that neural progenitor cells (NPCs) that overexpress CEND1 display increased neuronal differentiation in a mouse model of brain injury, suggesting its potential use as a therapeutic intervention for neurodegenerative diseases and brain injury (5). Alternate Names BM88; BM88 antigen; cell cycle exit and neuronal differentiation 1; Cell cycle exit and neuronal differentiation protein 1; CEND; CEND1; FLJ90066; MGC34326 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG