CST, 75806SF, Hexokinase I (C35C4) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Hexokinase 1. Validated for WB,IHC,IF,IF,F. Highly specific and rigorously validated in-house, Hexokinase I (C35C4) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #75806) is ready to ship. Product Usage Information This product is the carrier free version of product #2024. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2024 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Hexokinase I (C35C4) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total hexokinase I protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu108 of human hexokinase I protein. Background Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3). Alternate Names brain form hexokinase; glycolytic enzyme; hexokinase; hexokinase 1; Hexokinase 1 isoform HKI variant; hexokinase IR; hexokinase type I; Hexokinase-1; Hexokinase-A; HK; HK I; HK1; HK1-ta; HK1-tb; HK1-tc; HKD; HKI; HMSNR; HXK1; NEDVIBA; RP79 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 102 Source/Isotype: Rabbit IgG