CST, 75885T, SNX17 (E8F8Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SNX17. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SNX17 (E8F8Y) Rabbit Monoclonal Antibody (CST #75885) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SNX17 (E8F8Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total SNX17 protein. Non-specific bands may be detected above 120 kDa in some cases. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn54 of human SNX17 protein. Background Sorting nexins (SNXs) are a family of cytoplasmic proteins characterized by the presence of a phosphatidylinositol 3-phosphate (PI3P) binding phox (PX) domain. This binding occurs mainly in the early endosome and allows for trafficking of the bound protein to either a degradative or recycling pathway (1). Sorting nexin-17, or SNX17, has been shown to preferentially drive trafficking of integrins, receptors, and a variety of other proteins away from degradative pathways (1). In addition to PX domain interactions, SNX17 also binds the NPxY motif on the cytoplasmic tails of lipoprotein receptors via its FERM domain (protein 4.1, ezrin, radixin and moesin). Some of these proteins include the low density lipoprotein receptor-related protein 1 (LRP1) and apolipoprotein E receptor 2 (ApoER2) (3,4). LRP1 is known to bind APP, regulating its processing and causing an increase in Aβ production, a known risk factor for AD. By binding APP in addition to LRP1, SNX17 recycles both proteins to the plasma membrane, maintaining normal cell surface levels of each (3). SNX17 acts similarly with ApoER2, facilitating trafficking and increasing recycling to the plasma membrane. This assists in regulating the binding of ApoER2 and reelin, an interaction that is known to be important for neuronal migration and the formation of brain structures in early development, as well as synaptic function, learning, and memory in the adult brain (4). Through these and other interactions, SNX17 has been shown to have a potential role in a wide variety of neuronal pathways and diseases. Alternate Names KIAA0064; SNX17; sorting nexin 17; Sorting nexin-17 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG