CST, 75912S, Cystatin-A (F2W9D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CSTA. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Cystatin-A (F2W9D) Rabbit Monoclonal Antibody (CST #75912) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Cystatin-A (F2W9D) Rabbit Monoclonal Antibody recognizes endogenous levels of total cystatin-A protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human cystatin-A protein. Background Cystatin-A (CST-A), also known as cystatin SN or cystatin-1, is a member of the cystatin superfamily of proteins. Its primary function is the reversible and competitive inhibition of cysteine proteases, particularly lysosomal cathepsins (e.g., cathepsin B, H, and L). This inhibitory action is crucial for regulating cellular protein turnover, tissue remodeling, and maintaining cellular homeostasis (1). Our understanding of the role that cystatin-A plays in disease is incomplete. Cystatin-A is reported to be upregulated in several solid tumors, and elevated expression has been associated with enhanced cell proliferation, migration, invasion, and poor prognosis (2-4), suggesting that it might act as an oncogenic driver. However, some studies reporting decreased cystatin-A expression suggest it may play a protective role in certain tumor types (5,6). Alternate Names AREI; CSTA; cystatin A; cystatin A (stefin A); cystatin AS; Cystatin-A; Cystatin-A, N-terminally processed; Cystatin-AS; CYTA; PSS4; stefin A; Stefin-A; STF1; STFA Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 11 Source/Isotype: Rabbit IgG