CST, 75952S, Goat Anti-Mouse IgG3, Fc gamma Specific Antibody (HRP Conjugate)

Secondary Antibody for studying in the research area. Product Usage Information Recommended Antibody Dilutions: 1:3000 - 1:5000 Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability. Storage Supplied in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease- Free) and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Goat Anti-Mouse IgG3, Fc gamma Specific Antibody (HRP Conjugate) detects the Fc portion of mouse IgG3 but does not cross-react with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins. This antibody conjugate has minimal cross-reaction with human, bovine, and rabbit serum proteins. This antibody conjugate may cross-react with immunoglobulins from other species. Species Reactivity: Mouse Source / Purification Goat Anti-Mouse IgG3, Fc gamma Specific Antibody (HRP Conjugate) is produced by immunizing goats with mouse IgG3. The Anti-Mouse IgG3, Fc gamma Specific Antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Background Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times. Specification REACTIVITY: M Source/Isotype: Goat