CST, 75959T, Phospho-Atg101 (Ser11) (E5Z5A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATG101 (Ser11) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Atg101 (Ser11) (E5Z5A) Rabbit Monoclonal Antibody (CST #75959) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Atg101 (Ser11) (E5Z5A) Rabbit Monoclonal Antibody recognizes transfected levels of Atg101 protein only when phosphorylated at Ser11. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser11 of human Atg101 protein. Background Atg101 was discovered as a binding protein for Atg13, a component of the ULK1 serine-threonine kinase complex required for autophagy (1-3). Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (4,5). It is generally activated by conditions of nutrient deprivation, but is also associated with a number of physiological processes, including development, differentiation, neurodegeneration, infection, and cancer (6). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. The ULK1 complex includes both Atg13 and FIP200 and is required for starvation-induced autophagy (7-9). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (1,2). Atg101 has been reported to be phosphorylated at Ser11 by ULK1 (10). Alternate Names ATG101; Atg13-interacting protein; ATGA1; autophagy related 101; Autophagy-related protein 101; C12orf44; chromosome 12 open reading frame 44; FLJ11773 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 25 Source/Isotype: Rabbit IgG