CST, 75984T, Phospho-NPM1 (Thr199) (E4C7S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NPM1 (Thr199) phosphate. Validated for WB,WB,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-NPM1 (Thr199) (E4C7S) Rabbit Monoclonal Antibody (CST #75984) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:6400 - 1:25600 Immunofluorescence (Immunocytochemistry): 1:6400 - 1:25600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 52911 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-NPM1 (Thr199) (E4C7S) Rabbit Monoclonal Antibody recognizes endogenous levels of NPM1 protein only when phosphorylated at Thr199. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr199 of human NPM1 protein. Background Nucleophosmin (NPM1), also known as B23, numatrin, or NO38, is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication, and molecular chaperoning activities (1,2). The gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM1 portion contributes to transformation by providing a dimerization domain, which activates the fused kinase (3,4). Alternate Names B23; MGC104254; NPM; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; Numatrin; testicular tissue protein Li 128 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Rabbit IgG