CST, 76010T, LEF1 (D6J2W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LEF1. Validated for WB,F,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, LEF1 (D6J2W) Rabbit Monoclonal Antibody (CST #76010) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Flow Cytometry (Fixed/Permeabilized): 1:100 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized), Chromatin IP, Chromatin IP-seq Specificity / Sensitivity LEF1 (D6J2W) Rabbit Monoclonal Antibody recognizes endogenous levels of total LEF1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu80 of human LEF1 protein. Background LEF1 and TCF are members of the high mobility group (HMG) DNA-binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1/TCF7), TCF3/TCF7L1, and TCF4/TCF7L2 (1). LEF1 and TCF1/TCF7 were originally identified as important factors that regulate early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers, including colon cancer (4,5). Alternate Names DKFZp586H0919; FLJ46390; LEF-1; LEF1; lymphoid enhancer binding factor 1; Lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF1-alpha; TCF10; TCF1ALPHA; TCF7L3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25-58 Source/Isotype: Rabbit IgG