CST, 76012S, mGluR2 (D7D8M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying mGluR2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, mGluR2 (D7D8M) Rabbit Monoclonal Antibody (CST #76012) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity mGluR2 (D7D8M) Rabbit Monoclonal Antibody recognizes endogenous levels of total mGluR2 protein. This antibody may cross-react with mGluR3. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular loops of mouse mGluR2 protein. Background Metabotropic glutamate receptor 2 (mGluR2) is a class C G protein-coupled receptor for the neurotransmitter glutamate in the mammalian brain. Unlike ionotropic receptors, metabotropic receptors do not form an ion channel pore themselves but are indirectly linked to ion channels (1). While mGluR1 and mGluR5 activate phospholipase C, mGluR2, mGluR3, mGluR4, and mGluR6 are coupled to the inhibitory G protein Gα(i/o) and inhibit adenylyl cyclase (AC) activity (1). Research studies have suggested that mGluR2/3 receptors may be potential targets for the treatment of Schizophrenia (2). Furthermore, mGluR2 interacts with the 5HT2A serotonin receptor to form a hetero-complex in the brain. This complex is a potential pharmacological target for hallucinogenic drugs (3,4). Alternate Names GLUR2; glutamate metabotropic receptor 2; glutamate receptor homolog; glutamate receptor, metabotropic 2; GPRC1B; GRM2; Metabotropic glutamate receptor 2; mGlu2; MGLUR2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100, >200 Source/Isotype: Rabbit IgG