CST, 76074SF, TIMP1 (D10E6) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TIMP1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, TIMP1 (D10E6) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #76074) is ready to ship. Product Usage Information This product is the carrier free version of product #8946. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8946 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TIMP1 (D10E6) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total TIMP1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala134 of human TIMP1 protein. Background TIMPs are members of the family of tissue inhibitors of matrix metalloproteinases (MMPs) that includes TIMP1, TIMP2, TIMP3, and TIMP4. The main function of TIMPs is their inhibitory effect on MMPs. TIMPs irreversibly inactivate MMPs by directly binding to their catalytic zinc cofactor and resultant inhibition of proteinase function (1,2). In addition to MMP inhibition, TIMPs have also been shown to interact with various membrane receptors on the cell surface. Some of these interactions include TIMP1 with CD63, TIMP2 with α3β1 integrin, and TIMP3 with VEGFR2, all of which result in distinct cellular effects (3). TIMPs are involved in a wide variety of biological functions, such as tumor angiogenesis and progression (4,5), wound healing, and vascular remodeling (6,7). Mutations in TIMP3 are associated with Sorsby's fundus dystrophy (8,9). Alternate Names CLGI; Collagenase inhibitor; EPA; epididymis secretory sperm binding protein; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; Metalloproteinase inhibitor 1; TIMP; TIMP metallopeptidase inhibitor 1; TIMP-1; TIMP1; tissue inhibitor of metalloproteinases; Tissue inhibitor of metalloproteinases 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 26 Source/Isotype: Rabbit IgG