CST, 76135T, Granzyme A (E4M9S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Granzyme A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Granzyme A (E4M9S) Rabbit Monoclonal Antibody (CST #76135) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Granzyme A (E4M9S) Rabbit Monoclonal Antibody recognizes endogenous levels of total Granzyme A protein. This antibody does not cross-react with human Granzyme B, H, K, or M proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu115 of human Granzyme A protein. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein (2). Granzyme A, or GrA, is a dominant mediator of toxicity , and is the most abundant protease in cytotoxic granules (3). Granzyme A cleaves substrate after Arg or Lys and can activate caspase-independent cell death pathways upon cleavage of the mitochondrial protein NDUFS3. Cleavage of NDUFS3 leads to reactive oxygen species (ROS) generation, which triggers translocation of the SET complex to the nucleus, where Granzyme A cleaves the SET complex and leads to the opening of DNA and DNA nicks (4-7). Alternate Names CTL tryptase; CTLA3; Cytotoxic T-lymphocyte proteinase 1; Cytotoxic T-lymphocyte-associated serine esterase-3; Fragmentin-1; GRAA; granzyme 1; Granzyme A; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); Granzyme-1; GZMA; H factor; Hanukah factor serine protease); Hanukkah factor; HF; HFSP Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG