CST, 7613S, Atg4A (D62C10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATG4A. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Atg4A (D62C10) Rabbit Monoclonal Antibody (CST #7613) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Atg4A (D62C10) Rabbit Monoclonal Antibody recognizes endogenous levels of total Atg4A protein. This antibody recognizes unidentified bands within the molecular weight range of 48-60 kDa which decrease with silencing of Atg4A expression. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Atg4A protein. Background Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg) (1). Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are widely conserved in eukaryotes (2). Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologs (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4) (3-5). The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homolog for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homolog is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homolog (6). Atg4A predominately cleaves GATE-16, athough it can cleave the other mammalian Atg8 homologues with lesser efficiencies (4,7,8). Mutation in the corresponding Atg4A gene is critical for redox regulation and inhibits autophagosome formation. Expression of this Atg4A mutation demonstrates a role for reactive oxygen species in nutrient-deprived autophagy (9). Alternate Names APG4 autophagy 4 homolog A; APG4A; ATG4 autophagy related 4 homolog A; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A; AUT-like 2 cysteine endopeptidase; AUT-like 2, cysteine endopeptidase; AUTL2; autophagin 2; Autophagin-2; autophagy related 4A cysteine peptidase; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; Cysteine protease ATG4A; hAPG4A Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 48-60 Source/Isotype: Rabbit IgG