CST, 76147T, Cleaved-IL-18 (Asp36) (F9I1L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL18 (Tyr37,N-term) cleaved. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved-IL-18 (Asp36) (F9I1L) Rabbit Monoclonal Antibody (CST #76147) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cleaved-IL-18 (Asp36) (F9I1L) Rabbit Monoclonal Antibody recognizes endogenous levels of mature IL-18 protein only when cleaved at Asp36. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp36 of human IL-18 protein. Background Interleukin-18 (IL-18), originally known as interferon-gamma inducing factor (IGIF), is part of the IL-superfamily of cytokines (1,2). This proinflammatory cytokine is synthesized as an inactive precursor which requires cleavage by Caspase-1 to become an active, mature molecule, which is secreted by monocytes and macrophages (3,4,6). It induces IFNγ production in T-helper-1 (Th1) cells and natural killer (NK) cells, and can regulate innate immunity via both Th1 and Th2 responses (4,5). Elevated IL-18 levels are associated with autoimmune disease, but may be balanced by binding to IL-18 binding protein (IL-18BP), which along with IL-18 neutralizing antibodies, are being examined in clinical trials (6,7). Alternate Names Iboctadekin; IFN-gamma-inducing factor; IGIF; IL 18; IL-1 gamma; IL-18; IL-1g; IL18; IL1F4; Interferon gamma-inducing factor; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; Interleukin-18; MGC12320 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG