CST, 76173S, CHMP2B (D4G3K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CHMP2B. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CHMP2B (D4G3K) Rabbit Monoclonal Antibody (CST #76173) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CHMP2B (D4G3K) Rabbit Monoclonal Antibody recognizes endogenous levels of total CHMP2B protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu173 of human CHMP2B protein. Background CHMP2B is a component of the ESCRT III (endosomal sorting required for transport complex III) complex (1, 2). The ESCRT system is composed of the ESCRT-0, -I, -II, and -III complexes, which function sequentially to direct the transport of ubiquitinated transmembrane proteins into the intralumenal vesicles (ILVs), which will eventually mature into multivesicular bodies (MVBs). CHMP2B is a homolog of yeast Vps2, which functions in the ESCRT-II complex to change the initial spiral-structure of snf7 into membrane-sculpting helices for the final pinch off process (3). CHMP2B probably functions similarly in mammalian cells. Research studies show that manipulation of the ESCRT-III complex leads to accumulation of CHMP2B at the plasma membrane and overexpressed CHMP2B polymerizes into a tight helical structure that deforms the shape of associated plasma membrane (4). Research studies have shown that mutation of CHMP2B is associated with frontotemporal dementia, (5, 6). Studies have further shown that the dysfunction of mutant CHMP2B expression may disrupts the normal endo-autophagosome and endo-lysosome pathways and lead to neurodegenerative diseases (6-9). Alternate Names ALS17; Charged multivesicular body protein 2b; CHM2B; CHMP2.5; CHMP2B; chromatin modifying protein 2B; Chromatin-modifying protein 2b; DKFZp564O123; DMT1; hVps2-2; Vacuolar protein sorting-associated protein 2-2; vacuolar protein-sorting-associated protein 2-2; VPS2 homolog B; Vps2-2; VPS2B Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG