CST, 76194S, FAT10 (D1Q3Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FAT10. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FAT10 (D1Q3Y) Rabbit Monoclonal Antibody (CST #76194) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FAT10 (D1Q3Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total FAT10 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FAT10 protein. Background HLA-F adjacent transcript 10 (FAT10/Ubiquitin D) belongs to the ubiquitin-like modifier (Ubl) family of proteins. The 18 kDa FAT10 protein contains two tandem Ubl domains that are oriented in a head-to-tail fashion and a free C-terminal di-glycine motif, which is available for isopeptide bond formation with target proteins via an E1-E2-E3 enzymatic cascade (1). Indeed, FAT10 provides a ubiquitin-independent signal for proteasomal degradation (2). Research studies have demonstrated that FAT10 expression is enriched in lymphoid organs and that its expression is transiently upregulated via the NF-kB pathway in response to pro-inflammatory cytokines such as TNFα and IFNγ (1,3-5). In solid tumors that possess inflammatory microenviroments, research studies have shown that FAT10 is overexpressed and may serve as a biomarker for inflamed tumors (3,4). Alternate Names Diubiquitin; FAT10; GABBR1; UBD; UBD-3; Ubiquitin D; Ubiquitin-like protein FAT10 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG