CST, 76229T, GILT (E3P1D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IFI30. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GILT (E3P1D) Rabbit Monoclonal Antibody (CST #76229) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GILT (E3P1D) Rabbit Monoclonal Antibody recognizes endogenous levels of total GILT protein. This antibody cross-reacts with a 170 kDa protein of unknown identity in some cell extracts. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly111 of human GILT protein. Background Gamma-interferon-inducible lysosomal thiol reductase (GILT) is synthesized as a precursor glycoprotein, which is proteolytically processed intracellularly into a mature lysosomal thiol oxidoreductase. Research studies have shown that GILT participates in protein thiol reduction within the endosomal pathway and may contribute to shaping the repertoire of epitopes that undergo MHC class II-dependent presentation (1-3). Additional studies have demonstrated that GILT serves as a restriction factor for multiple RNA-based viruses by inhibiting viral entry (4). Given the role of antigen presentation in facilitating an anti-tumor immune response, research studies have suggested that elevated GILT expression in multiple types of human cancers serves as a biomarker for improved survival and response to immune checkpoint blockade (5-8). Alternate Names Gamma-interferon-inducible lysosomal thiol reductase; Gamma-interferon-inducible protein IP-30; GILT; IFI-30; IFI30; IFI30 lysosomal thiol reductase; interferon gamma-inducible protein 30 preproprotein; interferon, gamma-inducible protein 30; IP-30; IP30; Legumaturain; MGC32056 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG