CST, 76291T, SPTBN1 (F8Y1H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SPTBN1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SPTBN1 (F8Y1H) Rabbit Monoclonal Antibody (CST #76291) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SPTBN1 (F8Y1H) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPTBN1 protein. This antibody detects a 50 kDa and a 70 kDa protein of unknown identity in some cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala2176 of human SPTBN1 protein. Background β-II spectrin (SPTBN1), the most common isoform of non-erythrocyte spectrin, is a cytoskeleton protein in many cell types (1). SPTBN1 is a large actin cross-linking and scaffold protein that links the cell membrane and cytoskeleton. It is crucial for not only cell shape and organization but also a variety of cellular functions, such as cell adhesion, spreading, cell cycle regulation, and apoptosis (1,2).SPTBN1 is involved in cell growth and development (particularly in brain) via the TGF-β/Smad signaling pathway (3,4). SPTBN1 knockout mice show defects in cortical organization, developmental delay, and behavioral deficiencies (3), and variants in human SPTBN1 have been strongly implicated in intellectual disabilities and autism (4). Aberrant expression of SPTBN1 in various cell and tissue types has been shown in cancer development and progression, particularly ovarian, breast, and liver cancers (5-8). SPTBN1 plays an anticancer role in many tumor types. It assists in chemotherapeutic resistance and decreases the migration ability of tumor cells (5,6), highlighting its role in metastatic tumor progression and its potential as a pharmaceutical target (7-9). Recent work has shown that SPTBN1 is important for controlling CAR-T cell migration targeting tumors (10). Alternate Names beta-fodrin; beta-G spectrin; Beta-II spectrin; beta-spectrin 2; beta-spectrin II; beta-spectrin non-erythrocytic 1; betaSpII; ELF; embryonic liver beta-fodrin; epididymis luminal protein 102; Fodrin beta chain; HEL102; Spectrin beta chain, brain 1; Spectrin beta chain, non-erythrocytic 1; spectrin beta, non-erythrocytic 1; spectrin, beta, non-erythrocytic 1; Spectrin, non-erythroid beta chain 1; SPTB2; SPTBN1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 270 Source/Isotype: Rabbit IgG