CST, 76335S, GRIM19 (F6I7Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GRIM19. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, GRIM19 (F6I7Y) Rabbit Monoclonal Antibody (CST #76335) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GRIM19 (F6I7Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total GRIM19 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GRIM19 protein. Background Gene associated with retinoic and interferon-induced mortality 19 (GRIM19), also known as nicotinamide adenine dinucleotide:ubiquinone oxidoreductase subunit A13 (NDUFA13), is a subunit of mitochondrial membrane complex I (1). GRIM19 expression is upregulated by IFN-β and retinoic acid, and in addition to its role in maintaining mitochondrial membrane potential, it antagonizes Stat3 to inhibit cell survival (1-3). In mouse models, due to its antagonism of Stat3, overexpression of GRIM19 has been shown to mitigate diet-induced obesity by promoting differentiation of adipocytes toward formation of brown adipose fat tissue rather than white adipose fat tissue (4). GRIM19 has also been found to act as a tumor suppressor and is downregulated in many cancers, including renal cell carcinoma, colon cancer, and several forms of lung cancer (5). In macrophages, GRIM19 knockdown was found to induce IL-1β secretion, enhance levels of NLRP3, caspase-1, and gasdermin D, and promote pyroptosis (6). Reduced GRIM19 levels were found in human chronic liver disease tissues, and deletion of GRIM19 in mouse liver elucidated a potential mechanism; deletion led to enhanced oxidative stress and NLRP3 activation, leading to hepatic fibrosis (7). Alternate Names B16.6; CDA016; Cell death regulatory protein GRIM-19; cell death-regulatory protein GRIM19; CGI-39; CI-B16.6; complex I B16.6 subunit; Complex I-B16.6; FLJ58045; FLJ59191; Gene associated with retinoic and IFN-induced mortality 19 protein; Gene associated with retinoic and interferon-induced mortality 19 protein; GRIM-19; GRIM19; MC1DN28; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; NADH-ubiquinone oxidoreductase B16.6 subunit; NADH:ubiquinone oxidoreductase subunit A13; NDUAD; NDUFA13 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 16 Source/Isotype: Rabbit IgG