CST, 76385S, Tox/Tox2 Matched Antibody Pair

Matched Antibody Pair for studying Tox2/Tox in the research area. Product Usage Information Matched Antibody Pairs consist of capture and detection antibodies that bind to non-overlapping epitopes. For specific identification of the capture and detection antibodies in this pair, please refer to the data figure caption. Optimal dilutions/concentrations should be determined by the end user. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. Storage Store at -20ºC. This product will freeze at -20ºC so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles . A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Background Thymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2). Tox plays a key role in T cell development in the thymus during positive selection (3-5). A study in Tox-deficient mice also revealed a requirement for Tox in CD4 T cell and NK cell lineage development, including NKT cells, FoxP3 T regulatory T cells, and lymphoid tissue-inducer (LTi) cells (6-8). Although Tox expression is primarily restricted to developing immune cells in normal tissues, Tox is induced by high antigen stimulation during chronic viral infection or cancer, regulating T cell persistence and exhaustion (9-12). Tox has also been shown to be aberrantly expressed in cutaneous T cell lymphomas (13-14). Alternate Names C20orf100; dJ1108D11.2; dJ495O3.1; GCX-1; GCX1; granulosa cell HMG box 1; Granulosa cell HMG box protein 1; granulosa cell HMG-box protein 1; KIAA0808; MGC15880; thymocyte selection associated high mobility group box; thymocyte selection-associated high mobility group box; Thymocyte selection-associated high mobility group box protein TOX; Thymus high mobility group box protein TOX; TOX; TOX high mobility group box family member 2; TOX1; TOX2 Specification REACTIVITY: H M R