CST, 76439S, Gasdermin B Antibody

Polyclonal Antibody for studying Gasdermin B. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Gasdermin B Antibody (CST #76439) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Gasdermin B Antibody recognizes endogenous levels of total Gasdermin B protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu96 of human Gasdermin B protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The gasdermin family, which includes GSDMA, GSDMB, GSDMC, GSDMD, and GSDME, has been shown to play a role in inflammation and cell death. Gasdermin D has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7). Gasdermin B (GSDMB) has been reported to be upregulated in several tumor types, and in breast cancer has been associated with metastasis and poor prognosis (8,9). In addition Gasdermin B has been associated with immune disorders including asthma (10,11). Gasdermin B expression has also be found in the lung epithelium associated with asthma. Gasdermin B can also have a role in pyroptosis as it was found to activate caspase-4 and promote Gasdermin D cleavage (12). Alternate Names gasdermin B; Gasdermin-B; Gasdermin-like protein; gasderminB-1; GSDMB; GSDMB-1; GSDML; PP4052; PRO2521 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 47 SOURCE: Rabbit