CST, 76536S, CARD14 (E3F8E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CARD14. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CARD14 (E3F8E) Rabbit Monoclonal Antibody (CST #76536) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CARD14 (E3F8E) Rabbit Monoclonal Antibody recognizes endogenous levels of total CARD14 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn96 of human CARD14 protein. Background CARD14 (caspase recruitment domain-containing protein 14) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, functioning as molecular scaffolds for the assembly of multiprotein complexes (1-3). CARD14 has been shown to regulate BCL10 function, leading to NF-κB activation (1-3). It also induces activation of p38 and JNK MAP kinase pathways via interaction with MALT1 (4). Full-length CARD14, CARD14fl or isoform 1, encodes both an N-terminal CARD domain necessary for NF-kB activation, and a C-terminal tripartite MAGUK domain (PDZ/SH3/GUK). A shorter isoform, CARD14sh, encodes the CARD domain but not the tripartite domain (1,2). Mutations of are associated with psoriasis and psoriatic arthritis (2,3). Alternate Names bcl10-interacting maguk protein 2; BIMP2; CAR14; CARD-containing MAGUK 2 protein; CARD-containing MAGUK protein 2; card-maguk protein 2; CARD14; Carma 2; CARMA2; caspase recruitment domain family member 14; caspase recruitment domain family, member 14; Caspase recruitment domain-containing protein 14; PRP; PSORS2; PSS1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120-140 Source/Isotype: Rabbit IgG