CST, 76647T, IRF-2 (E4F5B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IRF-2. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, IRF-2 (E4F5B) Rabbit Monoclonal Antibody (CST #76647) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity IRF-2 (E4F5B) Rabbit Monoclonal Antibody recognizes endogenous levels of total IRF-2 protein. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp252 of human IRF-2 protein. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). Structurally similar to other IRF family members, IRF-2 acts as both a transcription activator and repressor. IRF-2 promotes transcription of several genes, including histone H4, the VCAM1 cell adhesion protein, and CIITA, a MHC transactivator protein. Conversely, IRF-2 competitively binds to promoter sites utilized by IRF-1, repressing activation by this related transcription factor (3). Its activity may be controlled by inducible proteolysis (4). IRF-2 has also been associated with increased oncogenic potential (5,6) and lymphocyte development (7,8). Alternate Names DKFZp686F0244; Interferon regulatory factor 2; IRF-2; IRF2 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG